Under a national Expanded System on Immunization (EPI), a one-dose, single-antigen, live attenuated measles vaccine (MV) was used in a limited human population aged 8 weeks for a short period in Zhejiang province, China between the past due 1970s and early 1980s. Proportions of different dose of vaccine by age by vaccine Zileuton were also identified. Statistically significant variations between groups were assessed from the Chi-square test. Results Over 95% seroprevalence rates of measles were seen in all age groups except <7 weeks infants. Children aged 5C9 years were demonstrated lower seropositivity rates of mumps while elder adolescences and young adults were offered lower rubella seroprevalence. Especially, rubella seropositivity was significantly reduced female adults than in male. Nine measles instances were unvaccinated or unfamiliar vaccination history. Among them, 66.67% (6/9) individuals were aged 20C29 years while 33.33% (3/9) were babies aged 8C12 months. In addition, 57.75% (648/1122) individuals with mumps were children aged 5C9 years, and Zileuton 50.54% (94/186) rubella cases were aged 15C39 years. Conclusions A timely two-dose MMR vaccination routine Zileuton is recommended, with the 1st dose at 8 weeks and the second dose at 18C24 weeks. An MR vaccination speed-up marketing campaign may be necessary for elder adolescents and young adults, particularly young females. Intro Measles, mumps, and rubella are viral infections that are preventable through vaccination programs. Under a national Expanded System on Immunization (EPI), a one-dose, single-antigen, live attenuated measles vaccine (MV) was used in a limited KLF1 human population aged 8 weeks for a short period in Zhejiang province, China between the late 1970s and early 1980s. In 1985, the MV system was amended so that an additional dose could be given at 7 years of age. This routine was revised again in 2007, with the MV becoming replaced by a routine measles-containing vaccination providing a measlesCrubella vaccine (MRV) at 8 weeks of age, followed by a measlesCmumpsCrubella (MMR) vaccine at 18C24 weeks of age. Since 2008, revaccination policy has been implemented with MRV for the secondary school students. In 2010 2010, Supplementary Immunization Activity (SIA) was accomplished throughout the whole country. This large-scale measles vaccination marketing campaign was held on September, 2010, with providing a measles-mumps vaccine (MMV) to children aged from 8 weeks to 4 years old in the province. However, despite the safe, free, and high uptake rate of the two doses of measles-containing vaccine (MCV) and rubella-containing vaccine (RCV) and one dose of mumps-containing vaccine (MuCV), measles, mumps, and rubella remain common diseases throughout Zhejiang province. Measles outbreaks continued in Zileuton 2008, with 12782 instances reported, which translated to 252.61 per million of the population. From 2009 to 2011, the incidence of measles remained high at 3.14C17.2 per million of the population. Similarly, the incidence of mumps improved from 394.32 to 558.26 per million of the population in 2007 and 2008, respectively. Finally, the reported instances of rubella improved from 3284 to 4284 in 2007 and 2011, respectively, representing a 30.45% increase or an increase from 65.94 to 78.71 per million of the population. Therefore, the removal of measles and control of mumps and rubella are urgent general public health priorities in local areas. Serological surveillance can be effective in achieving these goals [1], [2]. In our study, we identified the incidence, seroprevalence and vaccination history of MMR in Zhejiang Province in 2011 to clarify the population immunity characteristics and aid in the development of improved vaccination strategies. Methods Study subjects A population-based cross-sectional monitoring study was carried out at two monitoring sites (Sanmen region and Cixi city) in healthy human population in Zhejiang Province between June and December 2011. The total of 16 towns within Sanmen region and 20 within Cixi city were stratified into 5 areas (east, west, north, south, and center), respectively. The 5 towns in each site were sampled from each region at random. At least 60 individuals within each selected towns were systematically sampled from your inhabitants register to be representative by age and gender. According to the plans and conventions on routine obligatory vaccination provided by the Ministry of Health of China in 2005, the sample.